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Dose-response DNA-damage in ear-canal hair-follicle cells from mobile-phone use
Turkey Created: 20 Apr 2018
ABSTRACT: The aim of this study was to investigate effect of radiofrequency radiation (RFR) emitted from mobile phones on DNA damage in follicle cells of hair in the ear canal. The study was carried out on 56 men (age range: 30–60 years old)in four treatment groups with n = 14 in each group. The groups were defined as follows: people who did not use a mobile phone (Control), people use mobile phones for 0–30 min/day (second group), people use mobile phones for 30–60 min/day (third group) and people use mobile phones for more than 60 min/day (fourth group).

Ear canal hair follicle cells taken from the subjects were analyzed by the Comet Assay to determine DNA damages. The Comet Assay parameters measured were head length, tail length, comet length, percentage of head DNA, tail DNA percentage, tail moment, and Olive tail moment. Results of the study showed that DNA damage indicators were higher in the RFR exposure groups than in the control subjects. In addition, DNA damage increased with the daily duration of exposure.

In conclusion, RFR emitted from mobile phones has a potential to produce DNA damage in follicle cells of hair in the ear canal. Therefore, mobile phone users have to pay more attention when using wireless phones.
KEYWORDS: Radiofrequency radiation, mobile phone, DNA damage, single strand break, ear canal hair follicle cells
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Source: Electromagnetic Biology and Medicine, Akdag M et al., 18 Apr 2018

Oxidative stress of brain and liver is increased by Wi-Fi exposure of rats during pregnancy and the development of newborns
Turkey Created: 8 Nov 2015
Abstract: An excessive production of reactive oxygen substances (ROS) and reduced antioxidant defence systems resulting from electromagnetic radiation (EMR) exposure may lead to oxidative brain and liver damage and degradation of membranes during pregnancy and development of rat pups.

We aimed to investigate the effects of Wi-Fi-induced EMR on the brain and liver antioxidant redox systems in the rat during pregnancy and development.

Sixteen pregnant rats and their 48 newborns were equally divided into control and EMR groups. The EMR groups were exposed to 2.45GHz EMR (1h/day for 5 days/week) from pregnancy to 3 weeks of age. Brain cortex and liver samples were taken from the newborns between the first and third weeks. In the EMR groups, lipid peroxidation levels in the brain and liver were increased following EMR exposure; however, the glutathione peroxidase (GSH-Px) activity, and vitamin A, vitamin E and -carotene concentrations were decreased in the brain and liver. Glutathione (GSH) and vitamin C concentrations in the brain were also lower in the EMR groups than in the controls; however, their concentrations did not change in the liver.

In conclusion, Wi-Fi-induced oxidative stress in the brain and liver of developing rats was the result of reduced GSH-Px, GSH and antioxidant vitamin concentrations. Moreover, the brain seemed to be more sensitive to oxidative injury compared to the liver in the development of newborns.
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Source: PubMed, Celik et al., 28 Oct 2015

Wi-Fi (2.45 GHz)- and Mobile Phone (900 and 1800 MHz)- increase Oxidative Stress in animals
Turkey Created: 14 Oct 2013
Abstract: The present study was designed to determine the effects of both Wi-Fi (2,45 GHz)- and mobile phone (900 and 1800 MHz)-induced electromagnetic radiation (EMR) on oxidative stress and trace element levels in the kidney and testis of growing rats from pregnancy to 6 weeks of age.

Thirty-two rats and their 96 newborn offspring were equally divided into four different groups, namely, control, 2.45 GHz, 900 MHz, and 1800 MHz groups. The 2.45 GHz, 900 MHz, and 1,800 MHz groups were exposed to EMR for 60 min/day during pregnancy and growth.

During the fourth, fifth, and sixth weeks of the experiment, kidney and testis samples were taken from decapitated rats. Results from the fourth week showed that the level of lipid peroxidation in the kidney and testis and the copper, zinc, reduced glutathione (GSH), glutathione peroxidase (GSH-Px), and total antioxidant status (TAS) values in the kidney decreased in the EMR groups, while iron concentrations in the kidney as well as vitamin A and vitamin E concentrations in the testis increased in the EMR groups.

Results for fifth-week samples showed that iron, vitamin A, and β-carotene concentrations in the kidney increased in the EMR groups, while the GSH and TAS levels decreased. The sixth week results showed that iron concentrations in the kidney and the extent of lipid peroxidation in the kidney and testis increased in the EMR groups, while copper, TAS, and GSH concentrations decreased. There were no statistically significant differences in kidney chromium, magnesium, and manganese concentrations among the four groups.

In conclusion, Wi-Fi- and mobile phone-induced EMR caused oxidative damage by increasing the extent of lipid peroxidation and the iron level, while decreasing total antioxidant status, copper, and GSH values. Wi-Fi- and mobile phone-induced EMR may cause precocious puberty and oxidative kidney and testis injury in growing rats.
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Source: PubMed, Ozorak et al, 08 Oct 2013

Oxidative stress induced by 1.8 GHz radio frequency electromagnetic radiation and effects of garlic extract in rats
Turkey Created: 1 Mar 2013
Abstract - Purpose: We aimed to study the oxidative damage induced by radiofrequency electromagnetic radiation (RF-EMR) emitted by mobile telephones and the protective effect of garlic extract used as an anti-oxidant against this damage.

Materials and methods: A total of 66 albino Wistar rats were divided into three groups. The first group of rats was given 1.8 GHz, 0.4 W/kg specific absorption rate (SAR) for 1 h a day for three weeks. The second group was given 500 mg/kg garlic extract in addition to RF-EMR. The third group of rats was used as the control group. At the end of the study, blood and brain tissue samples were collected from the rats.

Results: After the RF-EMR exposed, the advanced oxidation protein product (AOPP) levels of brain tissue increased compared with the control group (p < 0.001). Garlic administration accompanying the RF-EMR, on the other hand, significantly reduced AOPP levels in brain tissue (p < 0.001). The serum nitric oxide (NO) levels significantly increased both in the first and second group (p < 0.001). However, in the group for which garlic administration accompanied that of RF-EMR, there was no difference in serum NO levels compared with the RF-EMR exposed group (p > 0.05). There was no significant difference among the groups with respect to malondialdehyde (MDA) levels in brain tissue and blood samples (p > 0.05). Similarly, no difference was detected among the groups regarding serum paroxonase (PON) levels (p > 0.05). We did not detect any PON levels in the brain tissue.

Conclusions: The exposure of RF-EMR similar to 1.8 GHz Global system for mobile communication (GSM) leads to protein oxidation in brain tissue and an increase in serum NO. We observed that garlic administration reduced protein oxidation in brain tissue and that it did not have any effects on serum NO levels.
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Source: Informa Healthcare, Avci et al / Ondokuz Mayis University Department of Biochemistry, Nov 2012

Single-strand DNA breaks in human hair root cells exposed to mobile phone radiation
Turkey Created: 23 Feb 2012
Abstract Purpose: To analyze the short term effects of radiofrequency radiation (RFR) exposure on genomic deoxyribonucleic acid (DNA) of human hair root cells. Subjects and methods: Hair samples were collected from 8 healthy human subjects immediately before and after using a 900-MHz GSM (Global System for Mobile Communications) mobile phone for 15 and 30 minutes. Single-strand DNA breaks of hair root cells from the samples were determined using the 'comet assay'. Results: The data showed that talking on a mobile phone for 15 or 30 minutes significantly increased (p< .05) single-strand DNA breaks in cells of hair roots close to the phone. Comparing the 15-min and 30-min data using the paired t-test also showed that significantly more damages resulted after 30 minutes than after 15 minutes of phone use. Conclusions: A short-term exposure (15 and 30 minutes) to RFR (900-MHz) from a mobile phone caused a significant increase in DNA single-strand breaks in human hair root cells located around the ear which is used for the phone calls.
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Source: PUBMED, Prof. Olle Johanssen (via email), 23 Feb 2012

Dr Devra Davis Nobel co-laureate: "If the cellphone were a drug, it would be banned"
Turkey Created: 28 Aug 2011
Dr Devra Davis Nobel co-laureate: "If the cellphone were a drug, it would be banned"
Once carrying three cell phones with her, Nobel Prize-winning oncologist Devra Davis gave up using them after conducting a study over the dangers of these devices.

She says we allow our brain cells to die every time we speak on the phone and stresses that if the cell phone were a drug, it would be banned because it entered our lives without having been tested.

Highlighting that people face a big problem due to cell phones, the American oncologist suggests there are ways to ease the negative effects of our mobile telephones. The simplest precaution is to keep the cell phone far from our bodies and use an earpiece while using them.

Davis has waged a campaign to fight against the harms of radiation-spreading cell phones and recently wrote a book regarding the matter called “Disconnect.” “The truth about cell phone radiation, what the industry has done to hide it, how to protect your family,” reads the cover of the book.

The professor was in Turkey last week for a conference regarding the harms of the cell phones. In an exclusive interview with Today’s Zaman, she says she wrote “Disconnect” in order to get people to use cell phones properly.

Read the whole article here at link:
http://www.sundayszaman.com/sunday/newsDetail_getNewsById.action?newsId=249252
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Source: Agnes Ingvarsdottir

Abstract: Mice brain DNA change by genotoxic effect of Microwave radiation.
Turkey Created: 27 Jul 2011
The genotoxic effect of radiofrequency waves on mouse brain.
Mice brain DNA change by genotoxic effect of Microwave radiation, and as WE all know, our human brains can´t cope with it either.

Karaca E, Durmaz B, Altug H, Yildiz T, Guducu C, Irgi M, Koksal MG, Ozkinay F, Gunduz C, Cogulu O.
Department of Medical Genetics, Ege University Faculty of Medicine, Izmir, Turkey
emin.karaca@med.ege.edu.tr.

Abstract
Concerns about the health effects of radiofrequency (RF) waves have been raised because of the gradual increase in usage of cell phones, and there are scientific questions and debates about the safety of those instruments in daily life. The aim of this study is to evaluate the genotoxic effects of RF waves in an experimental brain cell culture model. Brain cell cultures of the mice were exposed to 10.715 GHz with specific absorbtion rate (SAR) 0.725 W/kG signals for 6 h in 3 days at 25°C to check for the changes in the micronucleus (MNi) assay and in the expression of 11 proapoptotic and antiapoptotic genes. It was found that MNi rate increased 11-fold and STAT3 expression decreased 7-fold in the cell cultures which were exposed to RF. Cell phones which spread RF may damage DNA and change gene expression in brain cells.
PMID: 21732071 [PubMed - as supplied by publisher]
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Source: Kalle Hallberg/Agnes Ingvarsdottir

Nobel laureate: If the cellphone were a drug, it would be banned
Turkey Created: 4 Jul 2011
Once carrying three cell phones with her, Nobel Prize-winning oncologist Devra Davis gave up using them after conducting a study over the dangers of these devices.
 
She says we allow our brain cells to die every time we speak on the phone and stresses that if the cell phone were a drug, it would be banned because it entered our lives without having been tested.

Highlighting that people face a big problem due to cell phones, the American oncologist suggests there are ways to ease the negative effects of our mobile telephones. The simplest precaution is to keep the cell phone far from our bodies and use an earpiece while using them.

Davis has waged a campaign to fight against the harms of radiation-spreading cell phones and recently wrote a book regarding the matter called “Disconnect.” “The truth about cell phone radiation, what the industry has done to hide it, how to protect your family,” reads the cover of the book.

The professor was in Turkey last week for a conference regarding the harms of the cell phones. In an exclusive interview with Today’s Zaman, she says she wrote “Disconnect” in order to get people to use cell phones properly.

“There is a misperception that if something is being sold, it is safe to buy it. This is wrong. I used to carry three cell phones with me, but after conducting a study about its harms, I gave that up. Sometimes, if I see a cell phone in my grandchild’s hand, I get upset. The microwave radiation between a cell phone and cell tower that is emitted when we do not use it is very harmful. The body and the brain absorb half of this microwave radiation. While we are speaking on the phone, because of the microwave radiation, our brain cells begin to die,” she says.

Davis notes that in an ideal world doctors would be consulted in the manufacturing process of technological products and added that the technology sector continues to ignore the fact that technological products enter our lives without undergoing tests regarding their impact on human health.

The professor underscores that people accept technological equipment which introduces radiation into their lives. “I don’t believe that there is a devil who constantly exerts an effort to produce cancer. The technology sector ignores the problems and we accept many things which bring radiation into our lives. The technology sector ignores the problems and acts as if there is no problem.”

In writing a book about the harms of cell phones, Davis acknowledges that it is not easy to get rid of the devices; however, users can at least try to use them properly. “If you carry your cell phone on your person, it means the danger is in your pocket. Only if it is turned off should you carry it on your person. In fact, this is written in all cell phone manuals, but no one reads and follows them. It is necessary to use an earpiece while speaking on a cell phone,” she warns.

Davis has friends who had brain cancer because of cell phone use. She dedicated her book to them. She says people have started to learn more about the dangers of cell phones and have begun to use them more carefully. “Some people working at CNN have given up using cell phones altogether,” she adds.

The scientific studies on cancer are well done in Turkey, according to the Nobel laureate. She praises a Turkish professor in this regard. “Turkish doctors are in a good position with regard to cancer research. If the world were a fair place, Professor Nesrin Seyhan of Gazi University would receive a Nobel Prize. I was really impressed by her research. As she is a Turkish woman, she could not get what she deserved. She is working on some very good projects at Gazi University. They are doing experiments on pregnant rabbits. They have exposed baby rabbits to cell phone radiation. They have observed damage to the rabbits’ eyes, brains and lungs. In fact, cancer is not the only harm caused by cell phones. It causes damage to DNA as well,” she explains.

Davis also comments on the cancer risks she observed during her stay in Turkey. The smoking ban in public indoor areas got a thumbs-up from the professor; however, many cancer risks caught her eye as well. “A basic problem in Turkey is that cell towers are very close to private homes and hospitals. I am very surprised to see that. When I saw Çaml&#305;ca, I was shocked. In this field, Turkey does not take Switzerland as its model. On this matter, the Swiss are in a very good position. Walking down the street, I saw obese children. This would create a very serious problem for future generations. Children are motionlessly sitting in front of the computer and eating fast food. Fast food culture has become widespread. This is something that we should avoid.”

In order to prevent cancer, Davis has adopted a number of habits. She exercises and swims every day. “No matter how old a person is, he or she should exercise. If they cannot play a sport, they should stretch their legs and arms. I swim every day. Even on a plane, I do certain movements. Deep breathing and stretching the body are very important. For this, I practice yoga,” she says. She also drinks plenty of water, and never smokes or consumes alcohol. Davis saw a toilet a la Turca, referring to Turkish-style toilets, which are different from toilets with seats, for the first time in her life. She found the a la Turca very sanitary.

“I avoid sugar and white flour. I never clean my house with chemicals; instead, I prefer lemon juice or vinegar. Even shampoos contain chemicals. I use castor oil for my hair and face. I do not use chemical cosmetic products either. I prefer natural cosmetics. Sleep is important; I make sure to get enough sleep. In order to get a good night’s sleep, you need to sleep in complete darkness. For this, you should have heavy curtains in your room. When a person sleeps in the dark, the body releases melatonin, the happiness hormone,” she notes among other tips to maintain a healthy life.

She also has a motto for being healthy: “Eat broccoli, exercise, sleep well and don’t worry.”

Caution! There is a danger in your pocket

*Use a speaker, a hands-free device or an earpiece.

*Try to use a landline whenever possible.

*Try not to carry a cell phone on your person.

*Beware of weak signals. When the signal is blocked, radiation emission increases as the phone works harder to establish contact.

*Keep cell phones away from children. Microwave radiation from cell phones affects children twice as much as adults. Pregnant women should keep phones away from their abdomen.

*Don’t sleep with a cell phone turned on and placed next to the bed or under a pillow.
Click here to view the source article.
Source: Sundays Zaman, RAHIME SEZGIN, 03 Jul 2011

New Studies Reveal Stunning Evidence That Cell Phone Radiation Damages DNA, Brain And Sperm
Turkey Created: 24 May 2011
New independent studies offer stunning proof that confirms findings from the Council of Europe pulsed digital signals from cell phones disrupt DNA, impair brain function and lower sperm count. A meeting convened by Environmental Health Trust, with the Turkish cancer society, and Gazi University, revealed new research on this important issue showing damage to DNA, brain and sperm. Dr. Devra Davis, a member of a Nobel Peace Prize team, chairs this meeting with Prof. Nesrin Seyhan , WHO and NATO advisor who is head and founder of Biophysics Department and Bioelectromagnetics Laboratory at Gazi University in Ankara and founder of the Non-Ionizing Radiation Protection (GNRP) Center.

Prof. Seyhan's work confirms and strengthens the finding, that just four hours of exposure to RF-EMF disrupts the ability of human brain cells to repair damaged genes. Other new important work from Australia shows damage to human sperm. "We are deeply concerned about what this could mean for public health," noted Prof Seyhan.

"This work provides a warning signal to all of us. The evidence justifies precautionary measures to reduce the risks for everyone of us," says Prof. Wilhelm Mosgoeller from the Medical University of Vienna who has led European research teams, who found that RF-EMF induce DNA breaks. New studies carried out by scientists in Turkey, Russia and Israel, have investigated a variety of biological effects triggered by cell phones. Two years after false accusations against scientists who described DNA breaks, now the recent results finally show, that exposure induced DNA breaks are real.

On May 23, 2011, a think-tank of experts organized by Gazi University and EHT convened at a workshop in Istanbul, Turkey to share explosive independent studies on this topic. Titled "Science Update: Cell Phones and Health," the workshop will be held in Cibali Hall at the Kadir Has University, which is located in Istanbul, Turkey.

The workshop is co-sponsored by leaders in cell phone safety, including the Environmental Health Trust, The International Commission on Electromagnetic Safety, Gazi University and Athens University. The workshop coincides with the launch of Dr. Davis's book, Disconnect: The Truth About Cell Phone Radiation, What the Industry Has Done to Hide It, and How to Protect Your Family (Innobook, 2011) in Turkish.

The workshop will attract an array of leaders in the field, who will be discussing underreported, highly thought-provoking research never investigated in North America. The invited speakers include Prof. Dr. Murat Tuncer, Head of Cancer Control Department at The Ministry of Health of Turkey; Dr. Lukas H. Margaritis, Professor Emeritus in the Department of Cell Biology and Biophysics at the University of Athens; Dr. Nesrin Seyhan, of Gazi University's Biophysics Department; Prof. Dr. Wilhelm Mosgoeller of the Medical University of Vienna; Dr. Annie Sasco, Director of Epidemiology at INSERM/National Institute of Health in Bordeaux, France; Dr. Lukas H. Margaritis, Professor Emeritus in Department of Cell Biology and Biophysics at the University of Athens; Dr. Siegal Sadetzki, Cancer & Radiation Epidemiology Unit, Gertner Institute, Chaim Sheba Medical Center, Tel- Hashomer 52621, Israel and Sackler School of Medicine, Tel-Aviv University, Israel and Dr. Devra Davis, from Environment Health Trust in the US.

Impact of Cell Phones to Reproductive Heath and Cell Death

Among the highlights of the conference a research team at the University of Athens will discuss how insect studies have demonstrated that acute exposure to GSM (Global System for Mobile) signals brings about DNA fragmentation in insects' ovarian cells, and consequently a large reduction in the reproductive capacity of the insects. Further studies demonstrated that long exposures induced cell death to the insects in the study.

Cell Phones Impact to the Fetus

Workshop attendee Dr. Adamantia Fragopoulou, leader of a team at the University of Athens, will share a finding about embryonic development taking place in the presence of a mild electromagnetic field. Throughout the gestation period, exposure to radiation for just six minutes a day affects the bone formation of fetuses. The team suggests that this is possibly caused by the interaction of cell phone radiation with crucial molecules and ions involved in embryogenesis.

Cell Phones and How it Impacts the of the Young Brain

Dr. Seyhan will share work performed at Gazi University demonstrating that the increasing use of cell phones and the increasing number of associated base stations are becoming a widespread source of non-ionizing electromagnetic radiation. This work suggests that some biological effects are likely to occur even with low-level electromagnetic fields. The team concluded that 900 and 1,800 MHz radiation levels is related to an increase in the permeability of the blood-brain barrier in young adult male rats. The rat's brains can be used to correspond to the brains of human teenagers. This research is motivating by the fact that children are increasingly heavy users of cell phones; at higher frequencies, children absorb more energy from external radio frequency radiation than adults, because their tissue normally contains a larger number of ions and so has a higher conductivity. They conclude limiting cell phone and cordless phone use by young children and teenagers to the lowest possible level and urgently ban telecom companies from marketing to them.

In addition, research from a team at the University of Athens will share their studies demonstrating that rats exposed to cell phone radiation were unable to remember the location of places previously familiar to them. This finding is of potentially critical importance for people, who heavily rely on spatial memory for recording information about their environment and spatial orientation.

Related news:
May 2011, Turkey: Conference hears 'overpowering' evidence on cellphone dangers
May 2011, France: Council of Europe report: The potential dangers of electromagnetic fields
Click here to view the source article.
Source: Medical News Today, 24 May 2011

Conference hears 'overpowering' evidence on cellphone dangers
Turkey Created: 23 May 2011
Cellphones disrupt DNA, impair brain function and lower sperm count, say scientists from Turkey, Russia and Israel, who present their results at a conference today in Turkey.

Professor Nesrin Seyhan, a World Health Organization and NATO advisor from Gazi University in Ankara says just four hours of exposure to RF-EMF disrupts the ability of human brain cells to repair damaged genes.

Other new work from Australia shows damage to human sperm, confirming a similar report from Northern Ireland last week.

"We are deeply concerned about what this could mean for public health," says Seyhan.

A research team at the University of Athens will also discuss today how acute exposure to GSM signals causes DNA fragmentation in insects’ ovarian cells, drastically reducing their reproductive capacity. Long exposures induced cell death, they say.

And a team from the University of Athens says that, in rats, exposure to radiation for just six minutes a day affects the bone formation of fetuses. They suggest that this may caused by the interaction of cell phone radiation with crucial molecules and ions involved in embryogenesis.

Meanwhile, new work from Gazi University using young rats suggests that some biological effects are likely to occur even with low-level electromagnetic fields. The team concluded that 900 and 1,800 MHz radiation levels is related to an increase in the permeability of the blood-brain barrier in young adult male rats.

And with children increasingly heavy users of cell phones, they say, there's a powerful argument for limiting cell phone and cordless phone use by young children and teenagers to the lowest possible level, and banning telecom companies from marketing to them.

"This work provides a warning signal to all of us. The evidence justifies precautionary measures to reduce the risks for every one of us," says Professor Wilhelm Mosgoeller from the Medical University of Vienna.
Click here to view the source article.
Source: TG Daily, Kate Taylor, 23 May 2011

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